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On site Protocol |
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Lay out the 40 meter transit line at site 1, Turn on YSI85, calibrate after 5min. |
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Transport field kit to terminus of first seine site; set up photography and weighing stations |
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Using the GPS unit, mark way points at the beginning and end positions of the transect. Transfer the points to the data sheet. |
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Seiners (with wader waist bands snug) should enter the water outside of the transect line to a depth of about 1 meter and stretch the net so the poles are 5 meters apart. With the poles in contact with the bottom and the 5 meter line taught, walk backwards dragging the net to the end of the transect line. |
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At the end of transect, the person with the outside pole should walk in an arc until the net is parallel to the shore drag the cod end of the net on to the beach and sort catch by species. Only fish are quantified. Put the most abundant species into the 20 gallon tubs. Each species should go into a different container.
For one individual of each species collected, complete a Fish Identification data log. Photograph using macro mode.
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When the seine is out of the water, begin hydrographic measurements about mid-depth of the transect. Using the YSI meter, measure: water temperature, salinity, D.O., % D.O. saturation. Hold the Enter Button for about two seconds to save the data in the meter's memory. Transfer the data to the data sheet.
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Measure and record the total length (mm) of each individual of each
species in groups of 30 individuals.
When a column on the data sheet is complete, transfer the individuals
to the photographing container, add a photo ID label, and photograph
in macro mode using HQ resolution.
Transfer the group to a dry weighing dish and mass. Record the mass
under the photo ID value on the data sheet. Return the group to the
water. Our sampling permit requires us to return sensitive species (see
permit sheet) back to the water as quickly as possible. We are not allowed
to collect.
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Repeat for two additional sites.
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